产品货号：
Z22663
中文名称：
NSF抗体
英文名称：
Anti-NSF Antibody
产品规格：
50μl|100μl|150μl
发货周期：
1～3天
产品价格：
询价
| 抗体名称 | Anti-NSF Antibody |
| 指标别名 | NEM sensitive fusion protein;NSF;SKD2;Vesicle fusing ATPase;Vesicular fusion protein NSF |
| 克隆性 | Polyclonal |
| 检验物种 | human,mouse,rat |
| 应用范围 | WB,IHC,ICC/IF,FCM,ELISA |
| 基因名称 | NSF |
| 抗体来源 | Rabbit |
| 抗体类型 | IgG |
| 免疫原 | E.coli-derived human NSF recombinant protein (Position:R4-Q636). |
| 实际分子量 | 82KD |
| 成分 | 500μg/mL antibody with PBS，0.02% NaN3,1mg BSA and 50% glycerol. |
| 纯化方式 | Immunogen affinity purified. |
| 浓度 | 500μg/mL |
| 产品形态 | 溶液 |
| 保存条件 | 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
| 背景资料 | N-ethylmaleimide-sensitive factor,also known as NSF,is an enzyme which in humans is encoded by the NSF gene.NSF is a homohexameric AAA ATPase involved in membrane fusion.NSF is ubiquitously found in the cytoplasm of eukaryotic cells.It is a central component of the cellular machinery in the transfer of membrane vesicles from one membrane compartment to another.During this process,SNARE proteins on two joining membranes (usually a vesicle and a target membrane such as the plasma membrane) form a tight complex.This aids fusion of the vesicle with the target membrane.It has been proposed that the role of NSF is to undo these SNARE complexes once membrane fusion has occurred,using the hydrolysis of ATP as an energy source,allowing the dissociated SNAREs to be recycled for reuse in further rounds of membrane fusion.This proposal remains controversial,however.Recent work indicates that the ATPase function of NSF does not function in recycling of vesicles but rather functions in the act of fusing vesicles with the plasma membrane. |
| Uniprot ID | P46459 |
| 推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot. |
| 基因名全称 | N-ethylmaleimide sensitive factor,vesicle fusing ATPase |
| 蛋白名全称 | Vesicle-fusing ATPase |
| 推荐稀释比 | Western blot (WB) 1:500～2000 Immunohistochemistry (IHC) 1:50～400 Immunocytochemistry/Immunofluorescence (ICC/IF) 1:50～400 Flow cytometry (FCM) 1～3μg/1×106 cells Enzyme linked immunosorbent assay (ELISA) 1:100～1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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